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A Review of Six Decades of Lateral Flow Immunoassay (LFIA) Development: From Clinical Applications and Metabolic Marker Detection to Its Pivotal Role in COVID-19 Diagnosis and the Development of Rapid Kits in Aquaculture
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Mohammad Jalil Zorriehzahra   , Mina Ziarati , Monireh Faeed , Parisa Mohammadisefat , Laleh Yazdanpanah Goharrizi * , Sana Yousefian Jezi |
| Department of Fishery Science, Microbiology, Agricultural and Natural Recourses Research and Education Center of Kerman. Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran , l.yazdanpanah@areeo.ac.ir |
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Abstract: (30 Views) |
Lateral Flow Immunoassay (LFIA) technologies, also known as immunochromatographic assays, have been extensively utilized over the past six decades for disease diagnosis. These systems enable the rapid detection of biological ligands in clinical and environmental samples. Their primary advantages include low cost, accessibility, rapid turnaround time, and ease of use without the need for sophisticated laboratory equipment. The appeal of these portable diagnostic tools stems mainly from their acceptable analytical sensitivity and specificity, coupled with the visual interpretability of results. Due to these features, LFIA has gained significant traction, particularly in developing countries and emergency medical settings. In Iran, alongside global advancements, opportunities for the refinement and expansion of this technology have emerged.
This article provides a structured review of the evolutionary trajectory, advantages, limitations, and modern applications of LFIA across various fields, including COVID-19 diagnosis. Furthermore, it highlights recent achievements in fisheries and aquaculture, such as the design and development of rapid diagnostic kits for major viral pathogens, including Viral Nervous Necrosis (VNN), Viral Hemorrhagic Septicemia (VHS), and Koi Herpesvirus (KHV). |
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| Keywords: Lateral Flow Immunoassay (LFIA), Rapid COVID-19 Diagnosis, Aquatic Pathogens, Viral Disease Detection, Rapid Diagnostic Kits |
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Full-Text [PDF 992 kb]
(33 Downloads)
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Type of Study: Review |
Subject:
Marine Medicine
Received: 2025/11/15 | Accepted: 2025/11/30 | Published: 2025/12/1
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