|
Optimization of a Combined Process for High-Purity Phycocyanin Extraction and Purification from Spirulina platensis
|
Halimeh Rajabi *   |
| Ph.D in Marine Biology, Abadan University of Medical Sciences, Abadan, Iran , halimeh.rajabi@gmail.com |
|
|
Abstract: (20 Views) |
Background and Aim: Phycocyanin, a blue photosynthetic pigment found in the microalga Spirulina platensis, is widely used as a natural colorant in the food and cosmetic–pharmaceutical industries and has been reported to possess multiple therapeutic properties, including antioxidant and anti-inflammatory effects. This study aimed to Optimization of a simple, cost-effective, and efficient method for extracting and purifying phycocyanin with high purity.
Methods: Spirulina platensis was cultured under standard conditions. Initial extraction of phycocyanin was performed using water followed by phosphate-buffered saline (PBS), and optimization steps included repeated centrifugation, ultrasonication, and treatment with chitosan/activated carbon, freeze–thaw cycles, ammonium sulfate precipitation, and membrane filtration.
Results: The results demonstrated that optimizing the extraction process through freeze–thaw cycles combined with chitosan/activated carbon treatment or ammonium sulfate precipitation, enables the production of high-purity phycocyanin. Moreover, the application of membrane filtration proves to be an effective approach for achieving further purification.
Conclusion: This study presents an efficient and adaptable workflow for the extraction and purification of phycocyanin, which can be selected and applied based on the intended objective—whether prioritizing purity or yield. The proposed process enables researchers to obtain high-quality phycocyanin rapidly, cost-effectively, and reliably for a wide range of |
|
| Keywords: Phycocyanin, Spirulina platensis, Extraction and Purification, Biomedical Applications |
|
|
|
Type of Study: Original Article |
Subject:
Marine Medicine
Received: 2025/10/6 | Accepted: 2026/01/19 | Published: 2026/09/20
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
