Background and Aim: For proper treatment and control of nosocomial infections, it is necessary to know the resistance pattern of pathogenic microorganisms. The aims of the present study were to compare phenotypic and genotypic detection of pseudomonas aeroginosa and to determine the frequency of antibiotic resistant isolates from clinical samples of burn unit of Payambar e Azam Hospital in Bandar Abbas in 2016.
Methods: Morphological, biochemical and genetic identification methods based on 16s rRNA sequencing were used. Kirby Bauer method was used to determine antibiotic susceptibility according to CLSI guidelines.
Results: Of 50 Pseudomonas isolates, 27% of isolates were from skin samples and 73% from wound samples. The results of antibiotic susceptibility pattern showed that 46.15, 15.38, 46.15, 23.08, 15.38, 15.38 and 38.46% of the tested isolates were resistant to ciprofloxacin, imipenem, piperacillin, ceftazidime, respectively. Amikacin, azithromycin and cefepime showed resistance. This pattern for wound-derived isolates was recorded as 80.56, 25, 80.56, 27.78, 16.67, 33.33, 41.67 respectively. The results of genetic identification of 5 isolates confirmed phenotypic ones although these results did not confirm phenotypic results for W23 isolate. Phylogenetic studies revealed that S11 isolate had exposed evolutionary divergency rather than other examined isolates.
Conclusion: The presented results showed high antibiotic resistance especially to ciprofloxacin and piperacillin among examined isolates and emphasized on application of effective antibiotics and their limited prescription. The necessity of genetic identification of Pseudomonas strains is also suggested.